NAME
gt-ltrdigest - Identifies and annotates sequence features in LTR retrotransposon candidates.
SYNOPSIS
gt ltrdigest [option …] gff3_file
DESCRIPTION
- -outfileprefix [string]
-
prefix for output files (e.g. foo will create files called foo_*.csv and foo_*.fas) Omit this option for GFF3 output only.
- -metadata [yes|no]
-
output metadata (run conditions) to separate file (default: yes)
- -seqnamelen [value]
-
set maximal length of sequence names in FASTA headers (e.g. for clustalw or similar tools) (default: 20)
- -pptlen [start end]
-
required PPT length range (default: [8..30])
- -uboxlen [start end]
-
required U-box length range (default: [3..30])
- -uboxdist [value]
-
allowed U-box distance range from PPT (default: 0)
- -pptradius [value]
-
radius around beginning of 3' LTR to search for PPT (default: 30)
- -pptrprob [value]
-
purine emission probability inside PPT (default: 0.970000)
- -pptyprob [value]
-
pyrimidine emission probability inside PPT (default: 0.030000)
- -pptgprob [value]
-
background G emission probability outside PPT (default: 0.250000)
- -pptcprob [value]
-
background C emission probability outside PPT (default: 0.250000)
- -pptaprob [value]
-
background A emission probability outside PPT (default: 0.250000)
- -ppttprob [value]
-
background T emission probability outside PPT (default: 0.250000)
- -pptuprob [value]
-
U/T emission probability inside U-box (default: 0.910000)
- -trnas [filename]
-
tRNA library in multiple FASTA format for PBS detection Omit this option to disable PBS search.
- -pbsalilen [start end]
-
required PBS/tRNA alignment length range (default: [11..30])
- -pbsoffset [start end]
-
allowed PBS offset from LTR boundary range (default: [0..5])
- -pbstrnaoffset [start end]
-
allowed PBS/tRNA 3' end alignment offset range (default: [0..5])
- -pbsmaxedist [value]
-
maximal allowed PBS/tRNA alignment unit edit distance (default: 1)
- -pbsradius [value]
-
radius around end of 5' LTR to search for PBS (default: 30)
- -hmms
-
profile HMM models for domain detection (separate by spaces, finish with --) in HMMER3 format Omit this option to disable pHMM search.
- -pdomevalcutoff [value]
-
global E-value cutoff for pHMM search default 1E-6
- -pdomcutoff […]
-
model-specific score cutoff choose from TC (trusted cutoff) | GA (gathering cutoff) | NONE (no cutoffs) (default: NONE)
- -aliout [yes|no]
-
output pHMM to amino acid sequence alignments (default: no)
- -aaout [yes|no]
-
output amino acid sequences for protein domain hits (default: no)
- -allchains [yes|no]
-
output features from all chains and unchained features, labeled with chain numbers (default: no)
- -maxgaplen [value]
-
maximal allowed gap size between fragments (in amino acids) when chaining pHMM hits for a protein domain (default: 50)
- -force_recreate [yes|no]
-
force recreation of hmmpressed profiles (default: no)
- -pbsmatchscore [value]
-
match score for PBS/tRNA alignments (default: 5)
- -pbsmismatchscore [value]
-
mismatch score for PBS/tRNA alignments (default: -10)
- -pbsinsertionscore [value]
-
insertion score for PBS/tRNA alignments (default: -20)
- -pbsdeletionscore [value]
-
deletion score for PBS/tRNA alignments (default: -20)
- -v [yes|no]
-
be verbose (default: no)
- -o [filename]
-
redirect output to specified file (default: undefined)
- -gzip [yes|no]
-
write gzip compressed output file (default: no)
- -bzip2 [yes|no]
-
write bzip2 compressed output file (default: no)
- -force [yes|no]
-
force writing to output file (default: no)
- -seqfile [filename]
-
set the sequence file from which to take the sequences (default: undefined)
- -encseq [filename]
-
set the encoded sequence indexname from which to take the sequences (default: undefined)
- -seqfiles
-
set the sequence files from which to extract the features use -- to terminate the list of sequence files
- -matchdesc [yes|no]
-
search the sequence descriptions from the input files for the desired sequence IDs (in GFF3), reporting the first match (default: no)
- -matchdescstart [yes|no]
-
exactly match the sequence descriptions from the input files for the desired sequence IDs (in GFF3) from the beginning to the first whitespace (default: no)
- -usedesc [yes|no]
-
use sequence descriptions to map the sequence IDs (in GFF3) to actual sequence entries. If a description contains a sequence range (e.g., III:1000001..2000000), the first part is used as sequence ID (III) and the first range position as offset (1000001) (default: no)
- -regionmapping [string]
-
set file containing sequence-region to sequence file mapping (default: undefined)
- -help
-
display help for basic options and exit
- -help+
-
display help for all options and exit
- -version
-
display version information and exit
REPORTING BUGS
Report bugs to https://github.com/genometools/genometools/issues.