NAME

gt-ltrdigest - Identifies and annotates sequence features in LTR retrotransposon candidates.

SYNOPSIS

gt ltrdigest [option …] gff3_file

DESCRIPTION

-outfileprefix [string]

prefix for output files (e.g. foo will create files called foo_*.csv and foo_*.fas) Omit this option for GFF3 output only.

-metadata [yes|no]

output metadata (run conditions) to separate file (default: yes)

-seqnamelen [value]

set maximal length of sequence names in FASTA headers (e.g. for clustalw or similar tools) (default: 20)

-pptlen [start end]

required PPT length range (default: [8..30])

-uboxlen [start end]

required U-box length range (default: [3..30])

-uboxdist [value]

allowed U-box distance range from PPT (default: 0)

-pptradius [value]

radius around beginning of 3' LTR to search for PPT (default: 30)

-pptrprob [value]

purine emission probability inside PPT (default: 0.970000)

-pptyprob [value]

pyrimidine emission probability inside PPT (default: 0.030000)

-pptgprob [value]

background G emission probability outside PPT (default: 0.250000)

-pptcprob [value]

background C emission probability outside PPT (default: 0.250000)

-pptaprob [value]

background A emission probability outside PPT (default: 0.250000)

-ppttprob [value]

background T emission probability outside PPT (default: 0.250000)

-pptuprob [value]

U/T emission probability inside U-box (default: 0.910000)

-trnas [filename]

tRNA library in multiple FASTA format for PBS detection Omit this option to disable PBS search.

-pbsalilen [start end]

required PBS/tRNA alignment length range (default: [11..30])

-pbsoffset [start end]

allowed PBS offset from LTR boundary range (default: [0..5])

-pbstrnaoffset [start end]

allowed PBS/tRNA 3' end alignment offset range (default: [0..5])

-pbsmaxedist [value]

maximal allowed PBS/tRNA alignment unit edit distance (default: 1)

-pbsradius [value]

radius around end of 5' LTR to search for PBS (default: 30)

-hmms

profile HMM models for domain detection (separate by spaces, finish with --) in HMMER3 format Omit this option to disable pHMM search.

-pdomevalcutoff [value]

global E-value cutoff for pHMM search default 1E-6

-pdomcutoff []

model-specific score cutoff choose from TC (trusted cutoff) | GA (gathering cutoff) | NONE (no cutoffs) (default: NONE)

-aliout [yes|no]

output pHMM to amino acid sequence alignments (default: no)

-aaout [yes|no]

output amino acid sequences for protein domain hits (default: no)

-allchains [yes|no]

output features from all chains and unchained features, labeled with chain numbers (default: no)

-maxgaplen [value]

maximal allowed gap size between fragments (in amino acids) when chaining pHMM hits for a protein domain (default: 50)

-force_recreate [yes|no]

force recreation of hmmpressed profiles (default: no)

-pbsmatchscore [value]

match score for PBS/tRNA alignments (default: 5)

-pbsmismatchscore [value]

mismatch score for PBS/tRNA alignments (default: -10)

-pbsinsertionscore [value]

insertion score for PBS/tRNA alignments (default: -20)

-pbsdeletionscore [value]

deletion score for PBS/tRNA alignments (default: -20)

-v [yes|no]

be verbose (default: no)

-o [filename]

redirect output to specified file (default: undefined)

-gzip [yes|no]

write gzip compressed output file (default: no)

-bzip2 [yes|no]

write bzip2 compressed output file (default: no)

-force [yes|no]

force writing to output file (default: no)

-seqfile [filename]

set the sequence file from which to take the sequences (default: undefined)

-encseq [filename]

set the encoded sequence indexname from which to take the sequences (default: undefined)

-seqfiles

set the sequence files from which to extract the features use -- to terminate the list of sequence files

-matchdesc [yes|no]

search the sequence descriptions from the input files for the desired sequence IDs (in GFF3), reporting the first match (default: no)

-matchdescstart [yes|no]

exactly match the sequence descriptions from the input files for the desired sequence IDs (in GFF3) from the beginning to the first whitespace (default: no)

-usedesc [yes|no]

use sequence descriptions to map the sequence IDs (in GFF3) to actual sequence entries. If a description contains a sequence range (e.g., III:1000001..2000000), the first part is used as sequence ID (III) and the first range position as offset (1000001) (default: no)

-regionmapping [string]

set file containing sequence-region to sequence file mapping (default: undefined)

-help

display help for basic options and exit

-help+

display help for all options and exit

-version

display version information and exit

REPORTING BUGS

Report bugs to <gt-users@genometools.org>.